久久在线视频,av中文字幕在线观看,桃色视频在线观看,国产一区二区三精品久久久无广告

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 02.03 人胰腺癌細(xì)胞

Panc 02.03 人胰腺癌細(xì)胞

簡要描述:CRL-2553 Panc 02.03 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-26
  • 訪  問  量:1881

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

ATCC® Number: CRL-2553™ Price: $417.00

Designations: Panc 02.03

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 11

D5S818: 12,13

D7S820: 9,10

THO1: 6

TPOX: 9,12

vWA: 17

Age: 70 years

Gender: female

Ethnicity: White

Comments: Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 28.2 hrs [50655]

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2553

來自ATCC細(xì)胞,所有出入庫細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測,確保細(xì)胞無污染,符合檢測合格標(biāo)準(zhǔn)。歡迎來詢價詳談

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
人妻avwww| 美女少妇内射性色| 国产欧美一区二区| 精品射精| 亚洲av无码专区在线观看亚| 日本韩国一卡二卡在线| 18国产精品白浆在线观看免费 | 五月开心深爱网| 国产AV无码专区亚洲AV软件| 色色色五月开开心五月| 欧美肥妇毛多水多BBXX| 久久美国精品黄片| 99精品视频69v精品视频| 色欲人妻天天| 成全动漫视频在线观看免费播放| 午夜性乱伦| 欧美激情首页| 国产精品无码AV电影| 国内精自视频品一2区| 色毛片,黃色毛片| 熟女丝袜自慰88AV| 本道精品在线播放| 欧美精品一二三区| 欧美交片一区二区| 麻豆国产96在线日韩麻豆| 国产传媒三级在线| 天天日天天草天天干| 国产欧美加勒比| 欧美日本黄色| 中文字幕A二区| 国产骚穴| 夜夜骑天天干人人操| 中文字幕久久三区| 亚洲四虎成人网站视频| 影音先锋影院麻豆| 4438xx| 小草免费在线视| 五月丁香啪啪综合激情| 成人免费一区二区三区| 搞黄刺激 遮挡| 色妞色综合久久夜夜|